Mineralogy Room: contains numerous collections of minerals and rocks, fossil combustibles, and variously polished and worked building materials. Of considerably interest is a collection of crystallographic models made of glass and wood
Geology Room I: its rich Tuscan geognostic-lithologic collection is organised according to the respective periods
Geology Room II: contains collections of copper and iron minerals from different European countries
Palaeontology Room: in it is a fine collection of plant and animal fossils, represented by more than 1050 exemplars
Botany Room I: contains numerous collections of graminaceous, leguminous and polygonaceous seeds for horticulture and silviculture. Collections of tea, coffee, cocoa, etc.
Botany Room II: contains a magnificent collection of mushrooms made of wax, numerous herbals (Antoir, Bardi), and a collection of 160 Brendel of Berlin models (flowers and other parts of plants)
Zoology Room: contains a collection of the most well-known animals belonging to the various orders of vertebrates and invertebrates, an entomological collection belonging to the Guicciardini family, a very precious collection of microscopic specimens, a collection of glass models of molluscs, echinoderms, and coelenterates; and, lastly, a collection of preparations made of wax that demonstrate diseases in vines and other agricultural products
Industry Room: contains a collection of industrial and manufactured products, for a total of more than six thousand titles described in the catalogue that are divided into three classes: Applied mineralogy: ancient and modern porcelain and majolica; artificial marble; glass; asphalt; mineral oils; mineral and above all metallic products; mineral colours and mineral-chemical products; Applied botany: timber for various industries and products; textile fibres, straw and paper; vegetal colours, chemical products extracted from vegetables used in industry and in medicine; fatty and vegetal oils; soaps; rubber and gum resins; Chinese and Japanese products, fabrics and embroideries; Applied zoology: fatty and animal-sugar materials; fragrant animal materials; musk, amber, guano and artificial fertilisers; glue and gelatines; wool, horsehair, bristles, feathers, ivory, fur, leather and tanned hide, silk and products, sponges and corals.